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With frequent samples to keep us focused, we were instructed at length by our guide, a pipe-thin woman dressed in funky hand-sewn clothes, on the origins of chocolate, its cultivation and, naturally, the virtues of Theo's environmentally conscious method.
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Glutamine Ra was determined by a bolus injection of C-glutamine followed by a period of frequent sampling to establish the decay-curve for the glutamine tracer.
This will be explored in future studies using more frequent sampling to capture the early rise in insulin levels after intake of a standard meal and by developing a mechanistic model to account for complete insulin-glucose homeostasis.
It would also have been beneficial with a longer total study period, thus including more than two housing periods, and more frequent sampling to explore how herds' status change over time with better precision.
The variation in concentrations during the period varied by a factor of 4, also stressing the importance of frequent sampling opposed to spot sampling in environmental surveys and risk assessments.
This may also be the case in our study, although a more frequent sampling protocol to measure progesterone might have improved the interpretation of our data.
This larger fraction of genes changing with gestation is rather similar to the one of maternal plasma proteins that we have previously reported to change with gestational age, using frequent sampling from early to term gestation41.
More frequent samples may be needed, especially at 14 to 18 weeks, to follow the transition from the slow growth phase to the more rapid growth phase.
Overall, our results demonstrate that early, deep, and frequent sampling is needed to investigate viral/host interaction during AHI, which could help identify prerequisites for prevention and cure of HIV-1 infection.
Frequent sampling is needed to obtain robust and precise parameter estimates.
More frequent samples may have improved the accuracy.
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