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Often, less frequent mutations are not found because the new methods of mutational analysis are more sensitive but only for the identification of specific known mutations 9, 28, 29 or because the same exons of a gene are not always investigated in different studies.
Among the most frequent mutations are those occurring in genes that are activated by interferon and in genes that play a role in regulating immune response.
These frequent mutations are termed somatic hypermutation.
The most frequent mutations are a loss of function of p53 protein, a tumor suppressor, or in the p53 pathway, and gain of function mutations in the ras proteins, or in other oncogenes.
Three of the less frequent mutations are apparently novel.
The most frequent mutations are located in codon 634, occurring in more than 60% of all genetically identified MTC.
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Several specific residues for frequent mutations were found and the mutational frequency of reductase domains was much higher than that of heme domains.
Neither the mutation mechanism nor the mechanism of oncogenesis of these frequent mutations is understood.
Typical FMCA results of the frequent mutations were shown in Figure 3B.
The most frequent mutations were K103N and M184VI.
Mitochondrial DNA deletions, depletion and frequent mutations were excluded.
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