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Taken together, the limiting dilution and molecular cloning sequencing results strongly suggest that PCR error was responsible for the subsequent frequent false positive detection of K65R by UDPS of subtype C samples.
In the wake of frequent false positive findings in behavioral genetics, replication of the current findings is warranted before firm conclusions can be drawn.
The most frequent false positive results were reported in cases of laryngeal papillomatosis, where the microvascular pattern could be misinterpreted as malignant neoplasia [ 5].
Despite its widespread use for monitoring therapeutic response in OvCa patients, CA125 is a poor marker for early diagnosis due to frequent false positive and false negative results.
First, since studies of multiple SNPs and multiple phenotypes create the potential for multiple comparison problems and frequent false positive results, it would be important to either correct for multiple comparisons or know the predicted false discovery rate.
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The exact weight the panel gave to these considerations is unclear, but one factor that was clearly relevant was the problem of frequent false positives when testing for a relatively rare condition.
In many cases without yield loss CSNT values were below the 250 ppm "low" threshold, indicating frequent false positives for N deficiencies: The tool often identified deficiencies when in fact there were none.
However, chest radiographs have high interobserver variability, leading to frequent false positives and negatives [ 12, 13].
Interobserver agreement on chest imaging is poor, leading to frequent false positives and false negatives [ 12, 13].
Under these conditions, weaker positive hits are often buried in the noise produced by frequent false positives.
Frequent false positives siRNAs complicate the analysis of genome-wide RNAi screens that is why it is important to identify the candidate off-targeted transcripts in primary screening data.
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