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Branching frequency was calculated as the average number of lateral branches per bud.
Plume frequency was calculated as an averaged normalised value representing 22 weeks in each wet season.
In the regular stage the wavelength of extrudates was measured, and corresponding frequency was calculated.
The MN frequency was calculated as MN%= Number of cells containing micronucleus Tota number of cells scored × 100.
Deletion frequency was calculated assuming Hardy-Weinburg equilibrium, as this is a dominant marker.
The recombination frequency was calculated and analyzed using MapManager QTXb06 (Manly et al., 2001).
Miniature postsynaptic current frequency was calculated from 10 s of recording using MiniAnalysis software (Synaptosoft, Decatur, GA, USA).
Sighting frequency was calculated as the ratio of surveys with sharks to the total number of surveys in each cell.
Transformation frequency was calculated as the ratio of the transformant number to the recipient (competent) cell number.
The induced break frequency was calculated by subtracting values obtained in untreated cells from values obtained in radiation-treated cells.
An increase rate of frequency was calculated as the ratio of after to before isoproterenol application in the same cell.
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