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A low to high frequency repeated stimulation protocol was performed, consisting of a set of 10 contractions at the following stimulation frequencies: 0.1, 0.5, 1.0, 3.0, and 10 Hz.
Following the baseline period, the TBS protocol was delivered to one pathway and consisted of five bursts (10 stimuli at 100 Hz) at 5 Hz (theta frequency), repeated four times at an interval of 20 s. fEPSPs were then followed for 1 h before TBS stimulation was delivered to the other pathway and fEPSPs were followed for another hour.
A major limitation of DCE-MRI is the complex imaging requirements, which include initiation of the dynamic sequence prior to the intravenous injection within the magnet, followed by high frequency repeated scanning, that may force lower spatial resolution or reduced coverage of brain regions.
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Individually, high frequency repeats make up between 1.5% and 11.5% of the length of neointrons in which they reside.
To assemble the N. bombycis genome, the illumina reads were assembled by BGI's de novo assembly software [ 42], which assembled unique and frequency repeat areas of genome.
Occurrence frequency of repeats were used as criterion to screen the H. brasiliensis repeat library, and repeats appearing more than 100 times in each preliminary assembly were retained and combined into a H. brasiliensis high frequency repeat library as the input for RepeatMasker (version 3.2.9) [ 62].
High frequency repeats (those having ≥40 occurrences among all new intron sequences) had an average AT content of 93% and just over two-thirds of these, including the 23 most frequent repeats, had 100% AT content.
Albumin1 gene sequences from Phelipanche were also searched with BLASTn against the NCBI nt database in order to search for high frequency repeats and mobile elements, but no such features were identified.
The abundance and AT richness of high frequency repeats help to explain the high AT content of new introns relative to adjacent exonic regions, which average only 54.7% AT content.
These results support the conclusion from the 15mer profile analysis that the copperhead has many homogeneous TE sequences compared with the more diverged and/or lower frequency repeats in the python.
The frequency of repeated batch operation was controlled by the measured fermentation redox potential.
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