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However, increasing the frequency of transition by increasing the concentration of effector is often limited by other detrimental side effects caused by the effector.
It should be noted that the appearance frequency of the closed ring and the frequency of transition between round and distorted closed rings were highly dependent on ATP concentration.
The frequency of transition cycles between round and distorted closed rings as assessed by SDCH increased with increasing [ATP] and reached a saturated value around 0.6 s−1 at high [ATP], almost half of the saturated value of ATPase activity per hexamer (~1 s−1).
With the increase of voltages, the occurrence frequency of transition events was greatly improved.
The secondary criteria was the frequency of transition to prescription of antibiotics to assess the transition from post-viral ARS to acute bacterial rhinosinusitis (ABRS).
In the strong SQ regime, the frequency of transition between the equidistant levels (for the valuen = 0), at fixed valuesa1 = 0.5aeandc1 = 2.5ae, is equal toω00 = 3.32 × 1013 s− 1, which corresponds to the infrared region of the spectrum.
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The frequency of "transitions" in methylation states between neighboring CpGs is calculated and a transition score is computed by dividing the number of transitions by the number of CpGs.
The frequency of transitions between the round and distorted closed rings was low at low [ATP] (Fig. 2b, bottom panel), and increased with increasing [ATP] (Fig. 2b, middle and upper panels, and Supplementary Figure 12).
However, the frequency of transitions between the round and distorted states measured at various [ATP] was rather best fitted with a simple Michaelis Menten equation with Km = 0.35 ± 0.13 mM (Fig. 2e; blue open circles); here the transition frequency is defined by the total number of transition cycles between round and distorted states divided by the total period of HS-AFM observation.
The frequency of transitions between round and distorted closed rings was 0.10 ± 0.10 Supplementaryntary Figure 16b), which was nearly the basal level of ΔN-TClpB measured in the presence of ATPγS (Fig. 2e), although the ATPase activity of E423A was reduced only by half of that of ΔN-TClpB (Fig. 4b).
The frequency of transitions among different macro-states is rather low.
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