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However, in many cases, the frequency of stem cell engraftment and the number of newly generated cardiomyocytes and vascular cells, either by transdifferentiation or cell fusion, appear too low to explain the significant cardiac improvement described.
In fact, it didn't include two of the most common, prostate and breast, because the authors weren't able to pin down the size of the stem cell compartment or the frequency of stem cell divisions in those tissues.
Although it is unclear if CFE assays performed on passaged BMSCs are an estimate for the frequency of stem cells, we nevertheless, did not see any significant differences after FePro labeling.
Based on the present observations, however, this does not necessarily indicate an increased frequency of stem cells but may reflect a higher proliferative index in the stem cell compartment.
The closest approximation to estimating the frequency of stem cells is derived from the primary colony forming efficiency (CFE) assay, which enumerates the number of Colony Forming Units-Fibroblast, approximately 1 in 5 of which are stem cells [16].
To tailor the model to be species-specific, information about the populations of transit amplifying cells, differentiated cells and in vivo dividing frequency of stem cells will be evaluated based on experimental results.
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The frequency of stem-break scars was equal at Belum and Krau but less than in Central Africa and greater than in the Peruvian Amazon where tapirs are the only megafauna.
Therefore, this methodology should accurately reflect the frequency of stem-like cells in stem-like cell derived spheres.
Aging decreases bone marrow cellularity and alters the frequencies of stem cells.
Two lines (NIL22 and NIL28) that maintained relatively high frequencies of stem cell termination over several backcrosses were selected for mapping and phenotypic analysis after BC5.
To check the cell cycle status of intestinal stem cells, a pulse of EdU was administered to assess the frequency of CBC stem cells in WT and K-rasG12D epithelia that enter the S-phase.
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