The highest frequency of shoot regeneration (42.5%) was observed on the MS medium supplemented with 2.0 mg/l BAP, 0.5 mg/l NAA and 0.5 mg/l Kin.
In this protocol, 2 times MS Fe-EDTA were supplemented into Murashige and Skoog (MS) medium to suppress the chlorosis during watermelon explants culturing, and the optimal preculture time (5 days) and coculture time (5 days) were screened out to improve the frequency of shoots regeneration.
High frequency of shoot regeneration from explant (ranging from 73.3% to 100%) was obtained in all the treatments, excluding the control where only one shoot induced.
Increased ARF2 expression enhances the frequency of shoot meristem termination in zll-1.
In the first year the frequency of shoot growth may be genetically controlled as application of fertilizer and protection from fire does not encourage significantly faster shoot growth.
Stem explants showed a moderate frequency of shoot generation at 30%, which was all indirectly from callus and only the explants of axillary buds reached a high level of shoot proliferation.
The extent of callus growth prior to visible shoot regeneration was also, on average, two months longer for P. trichocarpa Nisqually-1 compared to P. tremula x alba 717-1B4, and the frequency of shoot initiation per callus much less.
The periodic solutions in the vicinity of fundamental natural frequency are determined by means of shooting method; the stability of the periodic orbits are investigated by determining the well-known Floquet exponents of the perturbed motions.
These include the layout of the geophones, the frequency of the shot source together with ways of improving the signal to noise ratio.
Whereas in the direction of shooting a relative decrease of the frequency of occurrence of lead containing particles with the increase of the distance was observed, on the shooting person, that is, in the direction opposite to shooting, a relative increase of the frequency of occurrence of lead containing particles with the increase of the distance from the muzzle took place.
