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The highest frequency of shoot regeneration (42.5%) was observed on the MS medium supplemented with 2.0 mg/l BAP, 0.5 mg/l NAA and 0.5 mg/l Kin.
High frequency of shoot regeneration from explant (ranging from 73.3% to 100%) was obtained in all the treatments, excluding the control where only one shoot induced.
Increased ARF2 expression enhances the frequency of shoot meristem termination in zll-1.
The extent of callus growth prior to visible shoot regeneration was also, on average, two months longer for P. trichocarpa Nisqually-1 compared to P. tremula x alba 717-1B4, and the frequency of shoot initiation per callus much less.
Stem explants showed a moderate frequency of shoot generation at 30%, which was all indirectly from callus and only the explants of axillary buds reached a high level of shoot proliferation.
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Dropping like logs, they fall horizontally through the air and land on thick mats to mimic the speed and frequency of shooting water.
In this protocol, 2 times MS Fe-EDTA were supplemented into Murashige and Skoog (MS) medium to suppress the chlorosis during watermelon explants culturing, and the optimal preculture time (5 days) and coculture time (5 days) were screened out to improve the frequency of shoots regeneration.
Among the cytokinins tested, the greatest frequency (100%) of shoot induction and number of shoots (12.4) were obtained on the medium supplemented with 2.0 mg L−1 BA (Table 1).
Another major response to the shade was a marked shift in shoot architecture towards a lower frequency of long shoots and a higher frequency of medium shoots compared with fully exposed vines.
These include the layout of the geophones, the frequency of the shot source together with ways of improving the signal to noise ratio.
A recent study published by the Harvard Injury Control Research Center shows that the frequency of mass shooting is increasing over time.
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