(Frequency of sIPSC); INa.
Bath application of 5-HT (20 µM) increased amplitude and frequency of sIPSC in both genotypes (p<0.05) and these increases were higher in slices from KO than WT mice (fold increase; amplitude, WT: 1.33±0.09, n = 10, KO: 2.02±0.25, n = 12, p<0.05; frequency, WT: 1.44±0.05, KO: 1.84±0.15, p<0.05, when compared between genotypes) (Fig. 2A).
First, the frequency of sIPSCs and mIPSCs was significantly lower in GluR5−/− mice compared to wild-type mice.
50 150 spontaneous inhibitory postsynaptic currents (sIPSCs) events were randomly selected from each recording and total sIPSCs calculated according to f×Q, f is the frequency of sIPSCs and Q is the average value for charge transfer per sIPSC [8].
Upon bath application of ATPA (3 µM), the frequency of sIPSCs was significantly increased to 281.3±48.4% of control (from 6.1±0.7 Hz to 17.1±2.0 Hz, n = 7, P<0.01) (Figure 4A and B).
This was followed by an immediate increase in frequency of sIPSCs in only one out of six recorded host neurons lasting exactly the same time as the light exposure (Fig. 7A, C).
As shown in Fig. 4E and F, bath application of capsaicin (5 µM) did not alter the frequency of sIPSCs and had no effect on the amplitude distribution [n = 6].
The frequency of sIPSCs in NRT neurons from both WT and SSADH−/− mice was lower compared to thalamocortical neurons (compare Figs. 1A and 2A, and Tables 1 and 2), in agreement with previous studies in rodents [26], [27], [29], [30].
In contrast to sEPSCs, no changes in the frequency of sIPSCs were detected after acute cocaine re-exposure in vitro.
The average frequency of sIPSCs was higher in D1 cells from the NAcC/NAcS compared with D1 cells from DLS.
In this view, CB1R represents a target to attempt the partial rescue of the drastic decrease of the frequency of sIPSCs in Rac1N pyramidal cells.
