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Using a digital slide viewer (ImageScope v8.0, Aperio Technologies), slides were inspected after scanning and a number of preliminary analyses were carried out on a small number of cases to establish the optimal frequency of points and optimal size of the area required to accurately assess the PoT (data not shown).
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In this case, the data embedding capacity corresponds to the frequency of peak point.
The solid line and color of solid circles show the magnetic field line and frequency of point packets of the applied chorus element, respectively.
In contrast to previous observations made in FA-deficient chicken DT40 B cells, which demonstrated reduced levels of SHM [23], [26], a high frequency of point mutations in somatically mutated Ig genes of both, wild-type and FancG-deficient B cells was found (Table 1).
Our present results clearly indicate a significantly higher frequency of point mutations in the inner layer of the small intestine- the site containing cells that should have undergone the most rounds of cell divisions- than the outer part, containing mostly smooth muscle cells and some remaining crypts.
The overall frequency of point mutation of FBXW7 in human cancers is about 6% [ 3].
Moreover, colorectal cancers with a high frequency of point mutations displayed a comparatively stable karyotype [ 15].
Second, burden analyses revealed a high frequency of point mutations of SHANK3 in ASD cases [ 25, 26].
The frequency of point mutations, such as base substitutions or frameshifts, remains similar in both cell types.
Moreover, MMR deficiency is also known to boost the frequency of point mutations, namely in proto-oncogenes.
The frequency of point mutations at codons 12 and 13 of the c-K-ras gene has been determined in a panel of more than 400 human tumors.
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