The increasing frequency of resistant microorganisms represents a serious health problem.
A significant association was found between the frequency of these microorganisms and death (P = 0.0001), and also between the presence of multiresistance and death (Table 2, P = 0.001).
Thus, we used this PCR-microtiter plate hybridization assay and we tried to detect U. urealyticum, U. parvum, M. hominis and M. genitalium in semen specimens collected from male partners of infertile couples in Tunisia in order to determine the frequency of these microorganisms.
The aim of the present study was to carry out a small survey on the frequency of mycobacterial microorganisms detected by solid and liquid culture and by a tetraplex real-time PCR for Mycobacterium genus, M. avium subspecies and M. tuberculosis complex in gut associated lymphoid tissue (GALT) and caecal contents of apparently healthy rabbits at slaughter.
Perhaps the reason for the high frequency of Gram-positive microorganisms was a more restrictive policy on the use of antibiotics, a policy not in place during a previous study [ 16] on our ICU in 1988, when Pseudomonas aeruginosa was the predominant microorganism.
The mutation frequency of a biofilm-growing microorganism is significantly higher than that of its planktonic form; for P. aeruginosa, up to a 105-fold increase in mutability has been observed [ 44].
It is added to drinking water to reduce or eliminate pathogenic microorganisms and frequency of occurrence at the consumer's tap.
Data for this study were obtained over the course of seven months between July 2011 and January 2012, and therefore seasonal variations in the frequency of bloodstream infection and causative microorganisms could not be assessed.
Parallel testing for Chlamydia spp. was included to explore the frequency of co-infections with 2 microorganisms that are difficult to propagate and that can cause disease patterns that may be clinically indistinguishable.
