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The duration of time-spent and frequency of entry into the open- and closed-arms were measured.
The regulatory topology of the HipBA network sets a frequency of entry into persistence, which may be a strong indicator of the frequency with which adverse environments are encountered.
SD rats cross-fostered onto WKY dams also showed increased exploratory behavior when compared to SD rats reared by SD dams, reflected by decreased latency to enter the inner zone and increased frequency of entry into the inner zone of the open-field but not the elevated-plus maze, possibly because of the increased element of anxiety caused by the elevation of the plus maze.
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Time spent and frequency of entries into each zone was calculated using EthovisionXT software (Noldus).
During a 5-min test period we recorded the frequency of entries to the open and closed arms.
The evaluated variables were latency to the first entry into the white compartment, time spent in the white compartment, and frequency of entries into the white compartment.
The dependent variables were the frequency of entries into each arm pair, the time spent (s) in the open arm pair, and the number of fecal boli excreted.
The parameters taken into account were: frequency of entries in the open and closed arms; total time spent in the open and closed arms; number of defecations [ 20].
Frequency of entries and time spent in the open/closed arms was recorded online (Ethovision 3.1) and statistically assessed using paired two-tailed t-tests.
The parameters analyzed in each lateral chamber were frequency of entries, total duration (in seconds) and total distance traveled (in centimeters).
During the sociability session, all rats showed a marked preference for the stranger 1 chamber) regarding duration and distance (P < 0.000001), but not frequency of entries (Table 1).
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