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The QC maintains a low frequency of division, which reduces vulnerability to DNA damage [ 2, 3], and also specifies adjacent cells to become the stem cells that actively divide to renew themselves [ 6, 7].
Another character with a similar distribution is the frequency of division of the anterior vulval precursor cell P3.p, which is lower in species of the Elegans group and in C. sp. 14 [ 43].
(1) Label retaining studies (Foudi et al., 2009; Qiu et al., 2014; Takizawa et al., 2011; Wilson et al., 2008) conclusively established that the HSC pool comprises at least two compartments differing in their frequency of division.
It is the transit-amplifying cells that provide the number of cells required for homoeostasis of the tissue in which they reside, as endogenous somatic stem cells are normally considered to have a low frequency of division (replicative quiescence).
Since the proportions of LT- and ST-HSCs in G0 were identical, we hypothesized that the differences in expression of cell cycle genes and frequency of division observed between these two subsets reflected differences in their capacity to exit quiescence upon mitogenic stimulation.
The first compartment might be understood as stem cells and their direct progeny, which are characterised by a low frequency of divisions, the second one as the direct progenitors of mature cells, which are characterised by a high frequency of division.
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(3) Division frequency When the frequency of cell division per unit concentration of the AER-signal, f div, becomes higher, the limb bud shape becomes broader and shorter (see Figs. 7 and 8).
The fact that a fraction of stem cells of all lineages remained undivided suggested that morphologically similar cells indeed display variation in the frequency of cell division and not all cells in hydra undergo incessant cell division.
Interestingly, a double immunofluorescence analysis of T-IhNSC-derived progenitors (3div in FGF2) with anti-βIII-tubulin and anti-GFAP showed the sporadic occurrence of asymmetric divisions (n<0,1% over the total number of cell divisions, given a frequency of cell divisions = 0.87±0.092% over the total cell number) in the differentiating culture (Figure S3, panel B).
Thus we investigated the effect of relative frequency of cell division on the SAM pattern formation.
The greater sensitivity of the endoderm to Poc1 reduction probably reflects the earlier differentiation of the ectoderm layer, associated with a reduction of the frequency of cell division.
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