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The observed mutation frequencies (entries of F) are related to the tree T by the proportions of normal cells and tumor clones that define the mixture in each sample.
Given that clofazimine inhibited calcineurin activation in T cells, we next determined whether clofazimine affected the oscillation frequency Ca2+ entry in Jurkat T cells, which has been shown to be critical and selective for sustained activation of calcineurin and NFAT to drive cytokine gene expression [30].
The regulatory topology of the HipBA network sets a frequency of entry into persistence, which may be a strong indicator of the frequency with which adverse environments are encountered.
SD rats cross-fostered onto WKY dams also showed increased exploratory behavior when compared to SD rats reared by SD dams, reflected by decreased latency to enter the inner zone and increased frequency of entry into the inner zone of the open-field but not the elevated-plus maze, possibly because of the increased element of anxiety caused by the elevation of the plus maze.
We then recorded standard spatiotemporal factors for 5 min (i.e., the frequency of entries into the open arms and the closed arms was recorded, whereby "arm entry" was defined as moving the head into an open arm).
Similarly to headache frequency, the entries for days with medication intake during the previous 4 weeks were consistent with those for the past week (1.5 ± 3.0 vs 0.5 ± 1.2 days; Pearson correlation coefficient 0.58, p < 0.001).
Time spent and frequency of entries into each zone was calculated using EthovisionXT software (Noldus).
During a 5-min test period we recorded the frequency of entries to the open and closed arms.
The dependent variables were the frequency of entries into each arm pair, the time spent (s) in the open arm pair, and the number of fecal boli excreted.
Frequency of entries and time spent in the open/closed arms was recorded online (Ethovision 3.1) and statistically assessed using paired two-tailed t-tests.
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