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Neurons were considered GABAergic based on the following electrophysiological criteria: continuous fast firing without spike frequency accommodation upon depolarizing current injection, fast spontaneous firing frequency >10 Hz and an action potential half-width ≤1.0 ms.
(H ) Comparison of instantaneous firing frequency accommodation during a depolarizing step.
Left: box-plot chart showing the development of frequency accommodation in s-GoCs (blue) and ns-GoCs (green).
AHPs are important for various neurophysiological properties, most notably spike frequency accommodation (Madison & Nicoll, 1984; Storm, 1989).
They display a very high input resistance (~600 MΩ) and can sustain high firing rates with only modest spike frequency accommodation (Geracitano et al. 2007).
The receptor can also facilitate calcium entry through NMDA receptors (Mannaioni et al., 2001) and activates somatic calcium transients, thus affecting frequency accommodation in hippocampal synapses (Niswender and Conn, 2010).
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High concentrations of ryanodine also selectively reduce the sAHP and spike-frequency accommodation (Borde et al., 2000; Shah & Haylett, 2000).
Spike-frequency accommodation (SFA) ratio was calculated as the ratio of the third to fifth inter-spike interval in the first trace containing ≥6 spikes.
The dependence of spatial frequency on accommodation has been investigated extensively.
The PARTS/M specifically assesses the use of assistance and the frequency which accommodations, adaptations or special equipment is used.
These GP neurons may also be classified into three groups by various membrane properties, such as membrane sag induced by hyperpolarization activated inward current (Ih), rebound firing, spike accommodation, spike frequency adaptation and spike afterhyperpolarization [9], [10], [11].
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