Following the initial gate selection, the frequencies of cytokine+ cells were quantified by quadrant statistics applied on FSC/laser forward-scatter versus FL2/anti-cytokine-PE dot plots for monocytes and neutrophils and FL3/anti-CD16, CD8, CD8 or CD19 TC versus FL2/anti-cytokine-PE dot plots for NK-cells, T-lymphocyte subsets and B-cells.
The COG frequencies of HF10, HF4000, and Km3 were quantified by the number of ORFs, because these metagenomes were derived directly from fosmid library sequencing without assembly.
Annexin V+/PI+ (late apoptotic) and Annexin V+/PI− (early apoptotic) cells were quantified by the frequency of fluorescently labeled cells and statistical significance was assessed by the two-sample t-test (independent variable).
The effects of load upon gait kinematic parameters (stride length, stride frequency, stance phase, and swing phase) were quantified by analysis of footage taken at 100 frames per second using a Sony HDR-XR520 video camera.
The impacts of oral problems on daily life were quantified by using frequency and severity scores (scales from 1 to 3) for difficulty in carrying out 8 daily life performances (eating, speaking, cleaning mouth, sleeping, emotion, smiling, study, and social contact).
Bulk optical absorption (µa) and scattering (µ's) parameters were quantified by fitting spatial frequency domain reflectance (R d (f ) ) data with a modified frequency-domain diffusion model by using a reference phantom with known optical properties by using custom Matlab program [ 10].
The frequencies of IFNα and TNFα producing pDC were quantified by incubating cells during the last 3h of 5h cultures with 10µg/ml Brefeldin A (Sigma-Aldrich).
The contractile characteristics at high pulse frequency stimulation (i.e., 300 Hz) were quantified by assessing the time to peak tension from the start of the evoked contraction (TPT300).
The frequency of IFN- γ and CD107a expression in NKT-like cells were quantified by multicolor flow cytometry.
The PA measures were quantified by merging two variables, one estimating the weekly frequency of PA and the other recording the locations where the PA takes place.
