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Detection of variants and estimation of their allele frequencies were performed using next-generation sequencing of DNA pools.
An accurate assignment of the vibrational modes corresponding to absorption frequencies were performed using potential energy distribution (PED) in a frequency range of 0 3 THz.
Comparisons of the distributions of the allele, genotype and haplotype frequencies were performed using the χ2 test.
Comparisons between frequencies were performed using the χ test.
Statistical comparisons of these frequencies were performed using the chi-square test.
Allele and genotype frequencies were performed using Arlequin v. 3.1 software [ 24].
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The comparison between allelic and genotypic frequencies was performed using the χ2 test and the exact Fischer test.
A preliminary identification of the natural frequencies was performed using the free decay response of the structure (impact hammer test) with different added masses.
A global test of differentiation among samples based on haplotype frequencies was performed using the Arlequin program.
Correspondence analysis based on mtDNA and Y-chromosome haplogroup frequencies was performed using the POPSTR program [ 34].
Measurement of PC frequencies was performed using freshly isolated cells, whereas Bmems were measured after 5 days of PBMC stimulation in vitro.
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