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It is known that SNPs with smaller minor allele frequencies are estimates with a greater margin of error, i.e. var earray) is not constant for all SNPs.
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Null allele frequencies were estimated using FreeNA (Chapuis and Estoup 2007).
The rest of the subcarrier frequencies are estimated by interpolation.
In this paper, three formant frequencies are estimated.
The statistical justification is that allele frequencies are estimated with error.
The background frequencies are estimated from positions 18 23 in each read.
The equilibrium frequencies are estimated from the gene and pseudogene sequences, and are shared by both models of sequence evolution.
Equilibrium nucleotide frequencies are estimated directly from the alignments (corresponding to our HKY85:ρ model from fig. 3).
Allele frequencies were estimated for all loci.
The allelic frequencies were estimated by gene counting, and the genotypes were scored.
In this way, the resonant frequencies were estimated to be 520 and 25 KHz, respectively.
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