Sentence examples for french cell from inspiring English sources
British Sky Broadcasting, the satellite giant had reportedly been in the running to buy AOL's British business, along with French cell phone provider Orange.
The upshot: More than 60% of French cell phone owners have an unlocked phone that can work with any GSM carriers services, says Hazelton.
"In the United States, I was really treated as a scientist the moment I walked into the lab and not as a laborer," says Maryse Bailly, a French cell biologist at the Institute of Ophthalmology in London.
More than 30 others are accused in the case--mostly of poisoning--including noted French cell biologist François Gros, Fabius's scientific adviser in the 1980s, and AIDS epidemiology pioneer Jean-Baptiste Brunet.
Cells were harvested, resuspended in 50 mM Tris (pH 8.5), 50 mM KCl, 5 mM MgAc, 0.1% NaN3 and 300 µM PMSF, and lysed by three passages through a French cell press.
E. coli cells were harvested by centrifugation at 4000 g for 10 min and disrupted in the purification buffer (50 mM sodium phosphate buffer (pH 8.0), 300 mM NaCl) plus 0.2 mM phenylmethylsulfonyl fluoride and 10 mM imidazole using a French pressure cell, and cell debris was removed by centrifugation at 10 000 g for 30 min at 4 °C.
After three passages through a French pressure cell at 110 MPa intact cells and cell debris were removed by centrifugation (30 min at 13000× g).
Cells were disrupted by two passages through a French pressure cell at 18,000 psi, and unbroken cells were removed by centrifugation at 33,000× g at 4 °C for 25 min. 1 –5 ml of broken cells was layered on top of the 15/40% (w/w) discontinuous sucrose gradient 15% sucrose band using either a peristaltic pump or pipette.
The suspended cells were lysed using French pressure cell press, debris was pelleted by centrifugation at 14000 rpm, and Ni-NTA resin (Qiagen) were added to the cleared cell lysate to capture the 6 × His tagged proteins.
Cells were then lysed in a 35 mL French pressure cell operating at 11,000 psi., and the intact cells and cellular debris spun down at 43,000× g for 15 minutes.
Briefly, cells were lysed (using a French pressure cell) in lysis buffer containing 50 mM Tris pH 8, 300 mM NaCl, 2 mM TCEP, and Complete protease inhibitor cocktail tablets (Roche Applied Science, Indianapolis, IN).
