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The minimum heat transfer rates of secondary drying, primary drying and freezing stages were 716.79 W and − 195.17 W and − 670.71 W, respectively.
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This section included questions on the number of embryos frozen, stage at implantation, and whether fresh or frozen embryos were used for each cycle of treatment.
Brains were frozen on a temperature-controlled freezing stage, coronally sectioned (25 µm) on a sliding microtome, and stored in a solution of PBS containing 0.02% NaN3 at 4°C.
Thus, only the solvents and not the solutes crystallized during the freezing stage.
Approximately 30-μm-thick serial sections were cut using a microtome (Leica SM 2010R) connected to a freezing stage (Physitemp Inc, BFS-30 MP Controller).
The embedded carcass was put on the quick freezing stage (−25°C) in the cryostat (Bright Instrument Company Ltd., UK) for about 120 minutes.
Seizure severity was assessed based on Racine's scale [ 16] (i.e., stage 1: freezing; stage 2: head nodding and blinking; stage 3: unilateral forelimb clonus; stage 4: bilateral forelimb clonus and rearing; stage 5: falling).
In Experiment 1, total and progressive motilities did not differ among pre-freeze stages of cryopreservation (P > 0.05).
For the Hives, it's simultaneously freezing on stage together mid-song.
Don't freeze of stage fright on stage.
Strained epitaxial growth of Fe70Pd30-xCux with x = 0, 3, 7 is used to freeze intermediate stages during the martensitic transformation.
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