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Cells were immediately stored on ice, placed in a freezer and frozen over night at -80°C (freezing speed about 1°C/min).
For samples like larger cells or tissues, where the Golgi field is located in the perinuclear region, this technique is not suitable, as the freezing speed decreases rapidly from sample surface deeper into the bulk, inducing ice crystal growth (Studer et al. 2008).
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Thus, the response during owl attack dichotomized to freeze (speed = 0) and flee (high speed).
A plastic spider in a display case was enough to make me freeze, then speed-walk back to the entrance.
"There's not nearly enough money to do this," said state Sen. Doug LaMalfa, R-Oroville, who has written a bill to freeze high-speed rail funding.
News from Spain helped lift stocks after the debt-laden country said it was considering freezing pensions and speeding up a planned rise in the retirement age as it raced to cut spending and meet conditions of an expected international sovereign aid package.
Besides, freezing will be sped up by 15% in the case of using finned and ringed tubes.
Scenes frame by frame, significant displacements, low-frequency videos, freezing, and high-speed displacements are frequent problems in images captured with an on-board camera due to the complex dynamic of the quadrotors during indoor flight.
Faster shutter speeds will freeze motion, slower speeds will blur movement.
In a randomized single blind parallel study, we tested the efficacy of an auditory metronome on walking speed and freezing in Parkinson's disease (PD) patients with freezing gait impairment during their 'on' function.
Photograph: Daniel Garcia/ AFP/ Getty Images 12.34pm BST A fast shutter speed freezes the water on the face of Australia's Stephanie Rice as she competes in the women's 200m individual medley heats swimming event.
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