Sentence examples for freezing sliding from inspiring English sources

Briefly, P0 brains were sectioned at 20 μM with a cryostat, and P7 P21 brains were sectioned at 40 μM with a freezing sliding microtome (Leica).

Brains were cut at 50 μm on a freezing sliding microtome.

All samples were cut transversely at 30 μm on a freezing sliding microtome.

Either coronal or sagittal sections of 40 µm are cut using a freezing sliding microtome and processed as free-floating sections.

The spinal cord extending 5 mm rostral and 5 mm caudal to the center of the injury site were then cryoprotected for 1 day in 30% sucrose before being sectioned in parasaggital orientation at 30 µm, using a freezing sliding microtome.

Four adjacent series of coronal sections (40 μm) were cut on a freezing sliding microtome.

Briefly, frozen slides were fixed in Bouin's solution.

Briefly, frozen slides were thawed, fixed in 4% paraformaldehyde, then delipidated in chloroform, dehydrated in graded ethanols and acetylated in triethanolamine buffer.

For immunocytochemistry, frozen slides were defrosted at room temperature and washed with PBS.

For immunofluorescent staining, frozen slides were thawed, fixed in acetone, washed in PBS, and blocked with 10% normal human serum.

To this end, matrices were frozen in optimal cutting temperature (OCT) compound, and 10 μm sections were prepared using a cryostat and then placed onto frozen slides.