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Two-way ANOVA was used for analysis of freezing score and general anxiety behaviors (Treatments × Days).
These rats were naïve to freezing score measurements on Fear, R 1, 3 and 7 d to make the situation relatively simple.
Thus, subjects chosen as founders for the LE and HE lines were required to have a post-acquisition (pre-extinction) freezing score greater than 75%%.
Here, our data indicated that delayed extinction produced a stable depression on conditioned fear responses indicated by the decreased freezing score until 20 days after the extinction.
Naive rats received i.p. injection of MK-801 at the dose of 0.3 mg/kg or Veh injection had no change in baseline freezing score and general anxiety behaviors measured 40 min after the injection compared with the naive rats (Figure S1).
A threshold is then applied to the data to yield a percentage freezing score.
Similar(48)
The baseline freezing scores, measured in Box B, of Fear, Imme EXT, EXT, Ext + Veh, Veh + EXT, MK-801 + EXT, EXT + MK-801 (4 h) and EXT + MK-801 (12 h) groups were not significantly different (Figs. 1 and 2).
That there were no difference in the temporal change of freezing scores between immediate extinction and fear group supports the inefficiency of immediate extinction on the conditioned fear response.
One-way ANOVA (genotype) was performed on freezing scores at each time point for mice tested in the secondary screen and two-way ANOVA was performed (genotype by treatment) for conditioned taste aversion.
The auditory cue was then presented for three minutes, and freezing scored again.
In contrast, we showed wide-ranging variance in freezing scores following extinction, with males showing a bimodal distribution.
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