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The present study was designed with the aim of finding a reliable semen freezing protocol.
However, the intrinsic toxicity and complex freezing protocol of DMSO still remain as the bottleneck of the wide use for clinical applications.
Using ice-templating, scaffold pore size was controlled (80 600 μm) via the freezing protocol and solution composition.
In conclusion, such a faster freezing protocol of semen packed in MFP could be advantageously applied to P1-spermatozoa (P1-SF), while the rest of the ejaculated spermatozoa could still be used for production of conventional artificial insemination (AI) doses, thus allowing for a maintained routine management of commercially relevant stud boars.
We thank Lukas Neukomm for pLN178 and pLN180 plasmids, Gery Barmettler and Therese Bruggmann from the center for microscopy and image analysis at the University of Zurich for TEM sample preparation and Michel Labouesse at the IGBMC Strassbourg for the EM worm freezing protocol.
A badly designed freezing protocol might induce differentiation impacting on the quality of the stem cells.
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Our objective was to determine the usefulness of these preventive sperm freezing protocols.
For comparison of the qualities of different biological specimens, freezing protocols and viability analyses need to be standardised.
Both construction designs allow the control of the nucleation temperature and contribute to a further standardisation of freezing protocols.
To determine the effect of various freezing protocols on postthaw development and pregnancy rates resulting from transfer of human zygotes.
We conclude that viable MSCs from diverse tissues can be recovered after cryopreservation using a variety of freezing protocols, cryoprotectants, storage periods and temperatures.
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