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A temperature stage was used in conjunction with a light microscope to observe freezing of the samples.
Nevertheless, this finding indicates the need for early centrifugation and measuring or freezing of the samples.
The bead beater was vibrating at 6500 Hz for 40 s and 2 plus 2 cycles were performed separated by freezing of the samples on dry ice.
The phenomenon of superfusion on the freezing of the samples was observed to happen between (-11°C) and (-14°C), after which the samples reached (-20°C) at 0.83°C/ min [ 10].
The maximum four hour delay due to transportation from the study subjects to the laboratory in the freezing of the samples in -70°C may have lowered the proportion of Bacteroides detected (Salonen et al., personal communications).
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Rapid freezing of the sample followed by electron microscopy analysis performed at very low temperatures enables data to be gathered on native samples in the absence of a fixative.
Serum C-peptide requires separating the serum by spinning rapidly and subsequent freezing of the sample.
The microcontroller triggers the freezing of the sample by communicating with the EMpact-2 via an RS232 serial communication channel.
The analyses were adjusted for technical variability according to Dumeaux et al. (time for phlebotomy to freezing of the sample, RNA extraction date and microarray lot number).
Solutions to this dilemma include immediate snap freezing of the sample in liquid nitrogen or dry ice [ 27, 28], or storage in special whole blood preservation buffers.
The high percentage of qPCR positive specimens not detected by multiplex DPO PCR might have had a low target concentration or were degraded by re-freezing of the sample [ 4].
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