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The freezing of streams over longer periods could be a limiting factor, with similar effects on the access to food resources as drought.
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During this period, strong advances of the glaciers fronts and episodes of freezing of the main streams occurred.
There are also thousands of streams throughout the state; streams and lakes may be frozen from December to mid-April, however.
Early California prospectors used pans and their hands to find nuggets in freezing cold streams.
However, the extreme cold temperatures of winter freeze lakes and streams and keep water levels low for much of the year, which hinders exploitation.
The absence of streaming video.
He also had to shoot rapids in a kayak, something he had never tried before; float naked in a freezing stream; and not flinch when an enormous grizzly passed within inches of him.
For the low temperature data, crystals were quick-dipped into 1∶1 mixture of Paraffin and Paratone-N oil for cryo-protection and immediately flash frozen in gas stream of liquid nitrogen prior to data collection.
Crystals of BRD4-1 were grown in the presence of 1 mM ligand and 10% (v/v) DMSO from reservoir, harvested in cryoprotectant (reservoir containing 25% (v/v) ethylene glycol and 0.5 mM ligand), and flash frozen in a stream of nitrogen gas.
Here, in order to obtain phasing information, selenomethionine-substituted protein was purified and crystallized, selecting only the cubic crystal form 1. Crystals were frozen in a stream of cool nitrogen gas (100 K) and brought to the Diamond Light Source, station I04 (DLS) for X-ray diffraction data collection.
Crystals of 3FY-Brd4(1) were grown in the presence of 1 mM -JQ1 and 10% (v/v) DMSO from 0.2 M NH4C2H3O2, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350; harvested in cryoprotectant (reservoir containing 25% (v/v) ethylene glycol and 0.5 mM ligand); and flash frozen in a stream of nitrogen gas.
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