Sentence examples for freezing down from inspiring English sources
Although the hut seemed warm enough, it was usually freezing down by the floor.
Cells were left undisturbed for 2 days, and split twice in a 1∶4 ratio before freezing down stocks.
A series of transformation stocks were prepared by growing S. pneumoniae in competence media (THYE with 0.2% BSA, 0.2% glucose, and 0.02% CaCl2) at 37°C in a 5% CO2 incubator to an OD600nm of 0.4, and freezing down the cultures in 20% glycerol.
From both colonies, we started with 10 wild-type and 10 bem1∆ cultures, which we evolved for one thousand generations by serial dilutions, regularly freezing down a sample, resulting (due to contamination) in nine surviving bem1∆ lineages and seven wild-type lineages.
Nobody would find me, and I'd freeze down there.
"We froze down the stretch and didn't make plays," Weber said, visibly irritated.
On the same time we strongly suggest the further use of the same cell lines been transfected in this studies in Orthotopic Xenotransplantation Studies, as we best stable cells were frozen down already.
Aliquots were frozen down and thawed as needed for infections.
Correct clones were re-thawn, expanded and frozen down till their further use.
PBMCs were aliquoted and frozen down in liquid nitrogen for future use.
Mouse T cells were activated for 36 h, collected and frozen down at different time points post-infection.
