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EBs from different time points (day 6.5 to 10.5) were washed with PBS and embedded in OCT freezing compound (Miles Scientific, Cambridge, ML) after fixation in 4% paraformaldehyde for 1 hour at 4°C and cryoprotection in 20% (wt/vol) sucrose overnight.
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The fact of having frozen compounds the humiliation of the harassment itself, but what, Greer wonders, could she have done?
Another anti-freeze compound, recently discovered in an Alaskan beetle that can tolerate -60C, might prove more useful.
Last year, the Smiths lost nearly all their wheat to a late freeze compounded by too much rain.
Chondrogenic differentiation was assessed by embedding micro-masses in OCT compound, freezing, and cryosectioning (6 mm).
We then killed the mice after 24 h, eviscerated and fixed the tumours by freezing with O.C.T. Compound (Sakura Finetechnical Co. Ltd ,Tokyo, Japan).
Mesenteric arteries were isolated and embedded in tissue-freezing compound.
The drought, which has been compounded by freezing temperatures, has already pushed up the cost of some produce, including corn and beans.
When frozen credit markets compound the risk that falling household wealth will cause a self-reinforcing cutback in personal consumption, production, employment and incomes, thereby plunging the American economy, and, conceivably, the entire world economy, into a costly, perhaps long-lasting recession?
The target was a saline solution of EGFR or EGR, the guest compound, frozen by thermal contact with liquid nitrogen.
To qualitatively measure the distribution of the GNPs as well as to provide a measure of MCL growth characteristics, MCL inserts were frozen in OCT compound for sectioning.
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