Sentence examples for freezing and heating from inspiring English sources

For this, the cells were inactivated by the process of freezing and heating.

MLVs (at a final concentration of 30 mM) were subjected to three cycles of freezing and heating to 60°C for 10 min for MLV homogenization.

His + transformants were selected on YPD plates containing Geneticin 418 (2–6 mg/ml), and then lysed by a combined enzyme, freezing, and heating treatment according to a simple protocol reported in the literature [ 31].

The measurement range was between − 196 and 600 °C, and the freezing and heating error was, respectively ± 0.1 and ± 2 °C.

Acoustic tests were conducted with differing levels of fine aggregate replacement with crumb rubber (7.5% and 15%) with four different grades following freezing and heating.

In this way, 94.8 g/L GABA was produced by concentrated XL1-Blue cells overexpressing GadB in 48 h (Park et al. 2013), and 280 300 g/L GABA was produced in 35 h by concentrated BL21 DE3) cells overexpressing GadA and treated with freezing and heating (Plokhov et al. 2000).

Such false-positive reactions can also occur when testing "fresh" sera because of a phenomenon that occurs in samples that are not frozen and heat-inactivated, as was suggested for the BoHV1 gE ELISAs [ 16].

Over-expression of ScTPS1 and ScTPS2 under stress associated rd29A promoter provided protection against drought, salt, freezing, and heat stress (Miranda et al. 2007).

ABF/AREB family members are upregulated by various abiotic stresses, such as drought, salt, freezing and heat stresses and are involved in stress/ABA signalling in different plant species (Choi et al. 2000; Uno et al. 2000; Yáñez et al. 2009; Hossain et al. 2010; Huang et al. 2010).

On the one hand, since the 1 m stratum is typically only exposed at extreme low tides, it does not experience the harsh conditions that the shallower intertidal strata are subjected to, e.g. desiccation, freezing, and heat, which may lead to lower species richness and biomass in the intertidal.

Temperature stresses include cold, freezing and heat.