For second-home owners who leave their furnaces on low to keep pipes from freezing in their absence, this means the system is continuously generating electricity that can be given back to the grid for a credit.
In order to freeze-dry the cells, we first had to develop a method that would enable freezing in the absence of permeating cryoprotectant agents (CPAs) such as DMSO, glycerol or ethylene glycol, to name a few, and would enable the use of additives that have a high glass transition temperature (Tg) and that are solid at room temperature.
Multimers were used either directly after multimerization, after storage at 4°C, or after freezing in the absence or presence of glycerol as indicated.
To measure the effects of these manipulations, we examine a behavioral assay of fear conditioning known as freezing (i.e. absence of movement in response to conditioning), either in the presence or absence of administered ketamine.
The presence or absence of freezing behaviour was scored every 8 s.
When higher concentration of alginate was utilized for cell encapsulation, the metabolic and angiogenic activity of the cells frozen in the absence of cryoprotectants was comparable to that observed in the presence of DMSO or trehalose.
Note that for the SEC-MALS experiment, protein was eluted with 35 mM sodium phosphate buffer pH 7.0, 1 mM MgCl2, 50 mM imidazole, 0.05 mM ATP and snap frozen in the absence of glycerol in 500 µl aliquots and stored in liquid nitrogen.
Transcriptomics-quality RNA can, in general, be isolated from buffy coats frozen in the absence of RNA preservative by thawing the samples in the presence of RNAlater, on condition that the buffy coats had been deep-frozen within 8 hr of blood collection.
supervised proceedings, observed for the presence or absence of gait freezing in patients with Parkinson's disease, monitored patient safety (including following discretely behind patients during trials in case of falls) and altered stimulation.
Gait freezing improved following electrode implantation in the absence of any stimulation in all patients, although this was less pronounced in patients implanted unilaterally.
