Sentence examples for freezer time from inspiring English sources
Both MMP-9 (r = 0.07, p = 0.02) and TIMP-1 (r = −0.10, p = 0.002) levels were statistically significantly albeit weakly related to freezer time.
Freezer time was a priori decided upon as a covariate in all models, as the effects of long-term freezing on these biomarkers is not known.
The samples had when thawed been frozen for a mean of 13.5 years (extremes 11.6 15.4 years), herein denoted "freezer time".
Serum levels of cathepin S was measured by enzyme-linked immunosorbant assay (human cathepsin S [Total], DY1183, R&D Systems) in frozen samples (mean freezer time 14.6 years [range 12.9 16.7]) (5).
The supernatants (the plasma) were transferred to new vessels and stored in −80°C freezer until time of use.
In addition, the questionnaire investigated the exposure to a home refrigerator and freezer at time of the study and in childhood.
First, the rate of enrichment of PSD proteins in the PSD fractions, tested in 12 subjects, appears to be relatively consistent between samples (Figure 2a), while the PMI of the subjects ranged between 3.5 and 22 h and the freezer storage time varied from 5 to 17 years.
We have previously verified sample preservation over freezer storage time [ 35], as have other researchers [ 83].
Systematic biases were minimized between the 1974 and 1989 laboratory analyses because the samples were measured concurrently, although freezer storage time was different.
Potential confounding variables (i.e. age, PMI, brain pH, freezer storage time, hemispheric laterality, antipsychotic drug exposure, sex and laterality) were evaluated with respect to the densities of PV mRNA+ and PV mRNA+/NR2A mRNA+ neurons, and vGluT1-immunoreactive boutons.
For both in situ hybridization and immunohistochemical experiments, we evaluated the effects of confounding variables, such as age, PMI, brain, pH, freezer storage time and exposure to antipsychotic medication (expressed as chloropromazine equivalent dose or CED) using analysis of covariance (ANCOVA).
