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Live specimens of all the study species were collected directly from nests in the field then placed immediately on liquid nitrogen for transport back to the laboratory, where they were held in a −80°C freezer pending genetic analysis.
The blood samples were labelled with a bar code, gently mixed and stored frozen upright in a -20 degree centigrade freezer pending DNA extraction.
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All samples were frozen in liquid nitrogen and stored in the freezer at -80°C pending total RNA extraction.
Leaves, bark, roots, embryogenic tissues (somatic embryos and callus) were frozen in liquid nitrogen and stored in the freezer at -80°C pending total RNA extraction.
Samples were then transported on ice to –80ºC freezers, where they were stored pending analyses.
During Part 2, all faecal samples voided whilst resident in the CRU were collected and stored in a freezer within 15 minutes of voiding pending analysis.
Instalments for her new freezer and her new washing machine, both of which are now lost between the ruins, are still pending.
Case pending.
Services pending.
Cases pending.
pending, Nos.
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