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Freezing and thawing was avoided since control experiments showed significant alterations in CD95hiCD45ROhi cell numbers when cells were stored in the freezer previous to immunostaining (data not shown).
The genomic inserts identified via sequencing of randomly picked clones prior to microarray experiments were among the most prominent regions identified, with the exception of the region of the gene encoding for topoisomerase IV subunit B, which was observed to grow slowly in the freezer stock recovery phase (data not shown).
The samples were concentrated under vacuum and kept in the freezer (− 80 °C) until NMR data acquisition.
To help vendors design better freezers in the future, the CEEL is collecting energy-use data on existing –80°C freezers.
To control the temperature in the freezer, the temperature sensors with automatic data logging were installed into the soil monoliths.
All saliva samples were kept in a freezer at −20 degrees Celsius until data collection was complete.
Data on the exposure to freezer confirm the association between CD and domestic refrigeration.
Pollen taken straight from the -20°C freezer gained weight for up to 14 hours (data not shown), presumably by absorbing water.
At the end of the data collection period, they were transported in that freezer to the Menzies School of Health Research laboratory in Darwin, Northern Territory and stored at −80°C.
The system simulation model was validated against experimental data obtained for a single-door 300-l vertical freezer.
Women stored the milk in their freezer until a research team picked up the sample during a home data collection visit later the same day.
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