Sentence examples for freeze medium from inspiring English sources

Semen samples were visually analyzed, frozen in liquid nitrogen either untreated or after addition of 0.33 mL SpermCryos Allround (SCA) freeze medium (ProVitro, Odense, Denmark) per 1 mL semen, and stored at −196°C until further analysis.

Each sample consisted of a cotton swab that had been rotated in the cloacae of a bird to obtain fecal material which was then inoculated into bacterial freeze medium (Luria broth; BD, Sparks, USA, phosphate buffered saline containing 0.45% Na-citrate, 0.1% MgSO4, 1% (NH4 2SO4, and 4,4% glycerol).

White colonies were picked from agar plates and grown overnight at 37°C in freeze medium.

The CPT was processed at the collection site according to the manufacturer's instructions and peripheral blood mononuclear cells (PBMCs) cryopreserved at 5 × 106 cells/ml freeze medium.

Each fecal sample, collected by swirling a cotton swab in a bird's cloaca or droppings, was submerged in a bacterial freeze medium and handled as described (5 ).

The lymphocytes were re-suspended in Cold Freeze Medium (60% RPMI 1640, 10% DMSO, 30% heat inactivated FCS) at a lymphocytes density of 6-12 × 106 cells/ml.

Monocytes were frozen for future use by suspending 10 × 106 cells/ml in ice cold freezing medium CTS® Synth-a-Freeze® Medium (Gibco).

The collected important organs were firstly fixed in 4 % neutral buffered formalin for 24 h, then dehydrated in 30%% sucrose solution, and processed into Tissue OCT-Freeze Medium and section.

OMCS were cryopreserved by storage in freezing medium (Cell Banker 1®) at −80 °C.

In Experiment 1, l-Glutamine from 20 to 80 mM was evaluated as a supplement for a conventional freezing medium for ram spermatozoa (Tris 20% egg yolk 7% glycerol).

hTGSCs were successfully cryopreserved without any change in their mesenchymal stem cell characteristics as they were treated with boron containing freezing medium.