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They starred in two syndicated television specials from a Canadian animation studio, Atkinson Film-Arts of Ottawa: The Care Bears in the Land Without Feelings (1983) and The Care Bears Battle the Freeze Machine (1984).
In honour of the Care Bears' 25th anniversary, another DVD edition of the film was released on March 20 , 2007 with restored picture quality; it contained the franchise's second Atkinson Film-Arts special, The Care Bears Battle the Freeze Machine, as an extra.
Early in their tenure, the Bears appeared as toys from the Kenner company, and starred in two syndicated television specials from a Canadian animation studio, Atkinson Film-Arts of Ottawa: The Care Bears in the Land Without Feelings (1983) and The Care Bears Battle the Freeze Machine (1984).
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So-called soft-service ice cream was invented in 1939; it is served directly from the freezing machine without being allowed to harden.
To monitor dynamic events and challenge the cell physiology, cells were maintained, treated and imaged on an inverted confocal microscope, directly on the rapid loader of the high pressure freezing machine (EMPACT-2, Leica Microsystems) which was placed on a tailored perfusion chamber (Fig. 1C-2).
Cryofixation of the suspension was finished in HPM 010 high Pressure Freezing Machine (TESCAN, USA).
Most researchers use either a programmable freezing machine or the more basic isopropanol "Mr Frosty" container to freeze their cells.
Briefly, small blocks from the CA1 region were frozen in a high-pressure freezing machine (HPM100; Leica Microsystems, Vienna, Austria) and fractured at −135 °C in a freeze-fracture machine (BAF060; Leica Microsystems).
Samples were then frozen by use of a high-pressure freezing machine (HPM 010; Bal-Tec, Balzers, Liechtenstein) and fractured by a double-replica method in a freeze-etching device (BAF 060; Bal-Tec).
3 × 3 mm areas of the coverglass containing STORM imaged sections were cut, rinsed in dH2O and frozen by abrupt application of the sample against a liquid helium cooled copper block with a Cryopress freezing machine.
Small blocks from the CA1 region were frozen in a high-pressure freezing machine (HPM 100, Leica Microsystem) and fractured at −135 °C in a freeze-fracture machine (BAF060, Leica Microsystem), as described previously (Masugi-Tokita et al., 2007; Lorincz & Nusser, 2010).
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