The show also features more idiosyncratic media, such as "Pig 05049," a book that tracks what happened to the components of a single pig after its death as they were used to make beer, bullets, anti-freeze, heart valves and chocolate as well as bacon, sausages and the book's own cover.
Rabbit for instance, admires the 'amazing protein perfect' of his little granddaughter's hair, and while driving past, thinks of Washington as 'the frozen heart, ice cream white, of the grand old republic.' Harry's own heart is not too good in fact.
In due time, that once frozen heart will be flowing gladly towards someone more deserving of you.
Sections (0.8 mm) of frozen heart were fixed in 2% paraformaldehyde, placed between two cover slips and digitally imaged using a Microtek ScanMaker 600z.
Cells or frozen heart sections prepared with OCT compound (Tissue-TeK 4583, Sakura Finetek Inc).
For immunohistochemical analysis, frozen heart section specimens were fixed with 4% buffered paraformaldehyde embedded in paraffin.
A histochemical azo-coupling reaction was performed on 5 μm frozen heart tissue sections to identify DPP4 enzymatic activity.
Fatty acid extraction and tissue triglyceride concentrations were determined on flash frozen heart tissue, liver tissue, and skeletal tissue as previously described [ 19].
Briefly, frozen heart extracts from old and young animals were thawed (50 μl) and subsequently assessed in phosphate-EDTA buffer containing GSH reductase (17 U/ml), NADPH (0.016 mg/ml) and DTNB (0.042 mg/ml).
For CD41 staining, frozen heart sections were labeled with anti mouse monoclonal antibody against CD41 (1 100 dilution, Abcam, Cambridge, MA), then incubated with FITC-conjugated secondary antibody (JacksonImmunoResearch Laboratories, West Grove, PA).
