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An iOS version of QualityTime is also planned — although, given Apple's control over its ecosystem — it's clearly not going to be able to take over the lock screen or freeze device usage.
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Therefore, in the multi-sample freezing device presented in this paper, the ice nucleus is produced in a separate volume of pure water inside an electrode cap.
Cells were then frozen using a directional freezing device.
In addition, we are up-scaling the MTG freezing device to allow for large-volume (between 20 and 150 ml of sample) directional freezing and freeze-drying.
Cells were then mounted on aluminum support disks and slammed onto a liquid helium-cooled copper block of a quick freezing device (Cryopress Med-Vac).
Cells were frozen to −80 °C using an isopropanol-filled, controlled-rate freezing device.
PBMCs were frozen using an automated controlled-gradient freezing device (Sy-Lab 14S-B, Neupurkersdorf, Austria) and then transferred into the vapor phase of liquid nitrogen.
For the selection of optimal cryopreservation media, uniform change in temperature during freezing and thawing, employed freezing device and long-term storage in liquid nitrogen are the indispensable factors to consider.
In freezing temperatures, this means ensuring the water trough has a heater or a no-freeze device, so the water never freezes over.
Improvement of this method towards a better resolution in the z direction at the light microscopy and a quicker fixation will require the coupling with recent super-resolutive light microscopy techniques and the development of novel high pressure freezing devices, respectively.
Specimens were rapidly frozen to contact with the pure copper block cooled by liquid helium by using the rapid-freezing device (Polaron, USA) and the frozen cytoplasmic surface was deeply etched and rotary shadowed with platinum/carbon and carbon, using the freeze-etching device (Bal-Tec BAF060, Liechtenstein).
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