Sentence examples for freeze cracked from inspiring English sources

He eliminated 10percentt of the city's 500 jobs, instituted a hiring freeze, cracked down on overtime and shifted 30 police officers from desk jobs onto the streets.

Small larval stages were freeze cracked after fixation and before RNAse treatment.

Four-day old adults (wild type or mutants) were grown from L1 on OP50, picked onto slides and freeze cracked.

Worms were decapitated with a razor blade in M9 buffer, freeze cracked on dry ice and fixed 2 min in methanol and 4 min in acetone at −20°C.

Sequencing of mutant alleles: 50 animals of the appropriate genotype were picked into 50µl of lysis buffer (50 mM KCl, 10 mM Tris pH 8.3, 2.5 mM MgCl2, 0.45% Tween-20, 0.01% Gelatin, 0.1 mg/ml Proteinase K) and freeze cracked in liquid nitrogen.

For mutant sequencing ~50 homozygous lethal animals were picked from the progeny of sDp3 balanced strains into 5 ul of lysis buffer (50 mM KCl, 10 mM Tris pH 8.3, 2.5 mM MgCl2, 0.45% Tween-20, 0.01% Gelatin) and freeze cracked in Liquid N2 followed by digestion (1 hour at 60°C followed by 15 min at 95°C). 1 ul of this DNA preparation was subsequently used in a 25 ul PCR reaction.

With this strange, elemental quality running through them, the people of "The Dark Dark" are not altogether different from the animals of "The Dark Dark," who are wonderfully spooky the dog that dies and then comes back to life, or the horse that walks calmly onto frozen, cracking ice.

That approach, central to some earlier attempts to slay inflation, leaves relative prices in disarray; when firms and workers try to restore them, the freeze cracks, and wages and prices start rising again.

Immunofluorescence of muscle myosin on embryos or adults was performed by freeze crack method as previously described (Wang et al., 2011) with the following adaptation for staining of adults: each mother was cut open in the middle using a sharp needle.

Its surface is frozen ice cracked by the massive tidal forces of Jupiter and nearby satellites.

(If you wait until frost, the water could freeze and crack the containers).