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It can freeze animals into inaction, which is hardly an effective defence tactic, and it can cause a variety of debilitating disorders, such as phobias, pathological anxiety and the increasingly fashionable diagnosis of post-traumatic stress disorder.Understanding how fear is formed in the brain may shed light on these disorders and help to develop ways to erase unwanted fears.
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Still, at least three companies have emerged in the last two years offering people the chance -- at a cost of hundreds or thousands of dollars -- to take that leap of faith and freeze animal tissue now so that some day they may be among the first to clone a pet.
These carvings are thought to have inspired CS Lewis to write the part in the Chronicles of Narnia where Aslan brings frozen animals back to life.
In the meantime, frozen animals are boozing it up to stay warm.
To estimate the total number of vesicle fusions per synapse, we froze animals 50 ms after stimulation when neurotransmission is complete.
This isolation used flash frozen animals extracted directly for DNA (without an oocyte preparation step), indicating that DNA cleavage is not due to the oocyte preparation procedure.
We froze animals 20 ms after stimulation and observed vesicles fusing along the plasma membrane; the diameter of these vesicles was 28.4 nm ± 0.3, the same size as a synaptic vesicle.
He spent many hours scouring the Siberian tundra, then trekking back to the site and using high-pressure water pumps to carve the frozen animal out of the ground and taking it back to an ice cave for storage and sampling.
The experiments were carried out by Perutz and his colleagues in a refrigerated meat locker in a Smithfield Market butcher's basement, behind a protective screen of frozen animal carcasses.
Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT).
Frozen animal tissue was disintegrated and analytical samples of 0.5, 1, 1.5, and 2 g were prepared.
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