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First, it is shown that commercial CFD software (CFX-5.3) can be used to adequately predict the particle-to-fluid heat transfer of a single free sphere.
Numerical tests show that the increase of computing costs needed to account for the free sphere degrees of freedom remains within about 20% per time step.
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Sampling of the void space with a spherical probe of 1.2 Å by using Voronoi decomposition 23 affords largest included spheres (Di) and largest free spheres (Df) with diameters of 23.4 and 22.0 [21.9] Å (Di) and 21.0 and 19.0 [19.1] Å (Df) for 1 and 2. The surface of these channels is decorated with imidazole, amino, amide and carboxylate groups from the peptidic backbone.
In this respect we have to distinguish between the value-free sphere of nature and the value-related sphere of culture (Rickert 1962, 21).
SKP cells were isolated from 38 skin samples by serum-free sphere culture and examined for the ability to differentiate into neural and mesodermal lineages.
Cells (10,000 cells/ml) were plated in suspension culture conditions in serum-free sphere media (DMEM F12 containing 0.4% BSA, 10 µg/ml insulin, 10 ng/ml EGF, 10 ng/ml FGF) on 6-well plates pre-coated with 1% agarose to prevent cell attachment.
The serum-free sphere culture medium consisted of DMEM/F12 (1 : 1) supplemented with 2% B27 (reagents from Invitrogen), 20 ng ml 1 recombinant human epidermal growth factor (Strathmann Biotech, Hamburg, Germany), 20 ng ml 1 leukaemia inhibitory factor (20 ng ml 1), and 10 IE ml 1 (5 μg ml 1) heparin (Roche, Mannheim, Germany).
Cells from four well-known cell lines derived from either squamous carcinomas (SiHa, Ca Ski, and C-4 I) or adenocarcinomas (HeLa) of the uterine cervix were cultured at low density (1,000 cells/ml) as suspension in serum-free sphere medium.
Felwick calls his meat-free spheres "veg balls", while Norman prefers the oxymoronic but more elegant sounding "vegetarian meatballs".
The zones obtained are the maximal singularity-free spheres or hyper-spheres for a given centre configuration within a given workspace.
Cells derived from nodular melanoma specimens were grown under serum-free sphere-forming conditions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com