Sentence examples for free shotgun from inspiring English sources
In this context, we investigated the effect of 10− 7 M Risedronate for 1 h and 48 h on MLO-Y4 osteocytic cells, through a quantitative, label free shotgun proteomic analysis.
Label-free shotgun proteomics is routinely used to assess proteomes.
Non-redundant proteins of 4,172 with a wide range of molecular weight (5.2 611 kDa) and pI values (pH 2.9 12.6) in developing and mature grains of rice has been identified by using a label-free shotgun technique (Lee and Koh 2011).
However, for the past decade, gel-free shotgun proteomics has become the major strategy for in-depth proteomic analyses.
Label-free shotgun proteomics in particular is highly effective for identifying peptides and for obtaining a global protein profile of a sample [ 7].
Therefore, the percentage of reads mapping to the mitochondrial genome (0.1%) in E. lengana can be considered to be on the high side for enrichment-free shotgun sequencing.
The 2D-DIGE strategy further solved the specific question of detecting differences between groups, a problem that faces even greater challenges in label-free shotgun metaproteomics.
This strategy could complement the extraction of SSU-rDNA genes from PCR-free shotgun metagenomic datasets, and, within these less taxonomically biased datasets, ease the selection of SSU-rDNA genes with sequence similarities to (groups of) divergent V4.
In preparation for gel-free shotgun proteomics, protein pellets were Lys-C and trypsin-digested using a modified method (A. Apffel, personal communication) of the Filter-Aided Sample Preparation (FASP) methods [ 84, 85].
The shift from gel-based to gel-free shotgun proteomics allows for high throughput and label-free quantitative comparison of biological samples, opening new research possibilities in plant sciences [ 28– 30].
Detection of proteins using label-free shotgun mass spectrometry fails to detect low abundance proteins in complex total extracts and allows only semi-quantitative estimation of relative protein amounts.
