The successful engineering of tomato chromoplasts for high-level transgene expression in fruits, coupled to hyper-expression of vaccine antigens, and the use of plant-derived antibiotic-free selectable markers, augur well for oral delivery of edible vaccines and biopharmaceuticals that are currently beyond the reach of those who need them most.
Innovative and different from more widely known local electromyostimulation, current WB-EMS equipment enables simultaneous activation of up to 14 18 regions or 8 12 muscle groups (both upper legs, both upper arms, buttocks, abdomen, chest, lower back, upper back, latissimus dorsi, and four free options) with selectable intensity for each region.
For these assays we generated a transgenic P. berghei parasite line that expresses a luciferase-GFP fusion protein and is free of a drug-selectable-marker [38].
By independent segregation of the chromosomes, selectable marker-free transgenic rice with the transgene only can be produced.
This mutant, which contains a disrupted b9 gene and is drug-selectable marker free, was used for deleting the slarp gene (PBANKA_090210).
In this case, T-DNA truncation along with independent segregation of multiple T-DNAs unintendedly resulted in a selectable marker-free segregant that is homozygous with regard to the gfp gene.
The truncation of some integrated T-DNAs at their left end along with the occurrence of independent segregation of multiple T-DNAs unintendedly resulted in a single-copy segregant that is selectable marker-free and homozygous for the gfp gene.
Transgenic P. berghei were generated either in wild-type strain ANKA 2.34 or in a selectable marker-free reporter strain of P. berghei ANKA cl15cy1 expressing the mu3 variant of green fluorescent protein clone RMgm-7 (Franke-Fayard et al., 2004).
Also beyond the cisgenesis framework it is not desired to introduce antibiotic resistance genes into the environment and in this study, we established a pipeline for Agrobacterium-mediated transformation of potato in the absence of a selectable marker gene (marker-free transformation).
The WEM-1 utilizes the 2.4GHz frequency range and four selectable channels to ensure interference-free .
