Sentence examples similar to free rigor from inspiring English sources

While high culture often demands the shock of the new, and mainstream home builders erect endless variations on faux traditional, he builds houses, civic buildings and the occasional monastery with the irony-free rigor of an ancient.

The heart (1.7 2.2 g in weight) was perfused for ∼10 h with the Ca2+-free rigor buffer.

The recorded fluorescence traces were compared with those obtained with "unchased" myofibrils treated in the same way as the chased ones but without adding the unlabeled cTn to the Ca2+-free rigor buffer.

To test to what extent changes in NBD-Tn fluorescence induced by mixing myofibrils with the activator Ca2+ reflect specifically rather than unspecifically bound NBD-Tn, the fluorescent NBD-cTn in exchanged myofibrils was "chased" by incubating the myofibrils for 1 h in Ca2+-free rigor buffer with an excess (10 mg/ml) of unlabeled cTn.

Before use, the cTn solution was thawed and then dialyzed (1 10 for 1 h at 4°C) against Ca2+-free rigor buffer containing 10 mM Tris, 132 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM NaN3, and 5 mM EGTA (pH 7.1 with NaOH).

To prevent overcontraction of the myofibrils after transferring them from the Ca2+-free rigor buffer (0 mM ATP) to the ATP-containing relaxation buffer, 10 mM of the actomyosin ATPase inhibitor BDM (2, 3-butandione-2-monoxime) was added before centrifugation of the myofibrils (380 × g for 10 min at 4°C).

To exchange the endogenous cTn in the myofibrils against NBD-cTn, the myofibril pellet was resuspended in Ca2+-free rigor buffer including protease inhibitors (0.5 mM 4- 2-aminoethyl benzenesulfonyl fluoride hydrochloride, 10 μM leupeptin, 14- 2-aminoethyl benzenesulfonylrotinin) and 1 mg/ml of NBD-cTn.

The lever-arm rotates to impel actin ending in the low free energy rigor state A.M. Cross-bridge repriming begins with ATP binding to A.M initiating dissociation from actin, ATP hydrolysis, and reversal of the lever-arm power stroke rotation.

While Zyuganov travelled on commercial flights with a small team of aides, Yeltsin was cosseted in the Presidential jet, free of the rigors of Aeroflot.

Being free from the rigors and demands of work, I have had time and the inclination once again to know my wife, to rekindle our love, to discover and rediscover the essence of the woman.

This includes a generous winter break stretching from roughly Thanksgiving to Valentine's Day — a pillow of time free from the rigors of weekly blog posts, in which reflection and study can return once again to their proper place at the center of any well-balanced life.