Sentence examples for free polymerase from inspiring English sources

A central idea of the hypothesis was from the reversible binding of CdTe QDs and free polymerase.

In many experiments, to exclude the possibility of generating mutations during the polymerization, we used the most error free polymerase available (15 26 fold better fidelity than ordinary Taq polymerase) AccuPrime™Pfx DNA polymerase and only 25 cycles.

But free polymerase does not do RNA synthesis so how can APRIL and pp32 bound to free polymerase influence an initiation process that occurs buried in the internal cavity of the polymerase which is part of a cRNP?

Yet in an infected cell vRNA synthesis occurs in the context of a cRNP particle, whereas the authors argue that APRIL and pp32 preferably binds free polymerase.

One can imagine the preparation is highly heterogeneous containing free polymerase, polymerase bound to the conserved 3' and 5' ends (which are protected from nuclease), nucleoprotein bound to RNA fragments, free nucleoprotein, etc.

After 2D classification, a large number of spurious particles as well as particles that show free polymerase or free clamp were removed, yielding a dataset of ∼90,000 particles.

The syn conformation of 1, N-ϵdG potentially allows Hoogsteen pairing with incoming dCTP, which might facilitate error-free polymerase bypass.

In this situation, as a result of ppGpp-deficiency due to the mutations, the transcription from the stress-responsive promoters can be impaired, partially due to the insufficient pool of free RNA polymerase core molecules that could bind to other σ factors related to stress tolerance, such as RpoN or RpoS (Potrykus and Cashel 2008).

Finally available and free RNA polymerase and ribosome numbers are set accordingly to represent average values.

They might either be influenced by transcriptional regulators that are expressed with the help of SigB or might be transcribed by another sigma factor and are therefore sensitive to the lowered amount of free RNA polymerase holoenzyme.

Figure 8(A) shows the free RNA polymerase (RNAp, s001) where, although the number of total molecules is fairly large (301 in this exemplar model), the state variable can only fluctuate between 301 and 297 molecules corresponding to zero or four polymerase molecules bound to the promoter and start sites of the two genes.