Sentence examples for free phosphate buffer from inspiring English sources
About 50 μl of peripheral blood was collected from the caudal vein of the specimens on each sampling time using heparinized 1-ml disposable syringes and transferred to 1-ml eppendorf tube containing 450 μl of chilled Ca++- and Mg++- free phosphate buffer saline.
5 × 106 cells (2 mock-transfected clones, pUSE-9 and pUSE-12; and 2 activated MEK1-transfected clones, H-MEK1-15 and H-MEK1-17) were suspended in calcium free phosphate buffer saline and subcutaneously injected on both sides of the SCID mice.
Tissue samples were collected in a sterile 15-ml centrifuge tube containing 1 ml of calcium and magnesium free phosphate buffered saline (PBS).
Following 3 hours of growth, the bacteria were pelleted at 5,900 g for 6 minutes and then washed two times with endotoxin free phosphate buffered saline (PBS).
Isolated islets were dissociated into single cell suspensions by careful pipeting after washing in 2 mM EDTA/PBS, and incubation for 10 min at room temperature in Ca2+ free phosphate buffered saline (PBS) supplemented with 0.025% trypsin.
For single cell studies of mitochondrial ATP levels, the islets were dispersed by incubation in Ca2+/Mg2+ free phosphate buffered saline, 3 mM EGTA and 0.002% trypsin as previously described [42].
and washed in cation free phosphate buffered saline (PBS).
To further explore the flat-band voltage drift effect and to challenge the assumption that alkali ions are involved in the drift we conceived a novel alkali-free phosphate buffer saline (AF-PBS) where the sodium and potassium ions are replaced by ammonium ion and tested the capacitor under similar conditions to standard PBS.
with 4×LD50 (4×104 p.f.u./ml) of PR8 virus (H1N1) in endotoxin-free phosphate buffer saline (PBS) under isofluran anaesthesia.
Samples weighing approximately 1 g were dissected, rinsed in RNAase-free phosphate buffer, snap-frozen in liquid nitrogen and stored at -80°C.
