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The biochemical assay is easy to apply but the cells need to be grown in media free of endogenous glucocorticoids, in order to prevent them from interfering with radiolabelled hormone binding to the receptor.
In our comparison of benign and carcinoma tissue extracts, we detected active MMP-2 and MMP-14 forms only in the cancerous tissue samples, indicating that a pool of the tumor MMPs is free of endogenous inhibitors (TIMPs), and is thus likely to contribute to proteolytic events that precipitate tumor metastasis.
Microtubules belong to those subcellular structures that are essentially free of endogenous actin, and they hardly associate with potentially interfering, cellular membranes along their entire length.
We here describe the ectopic nucleation of actin filaments on the surface of microtubules, free of endogenous actin and interfering membrane or lipid.
Sentinel mice were free of endogenous pathogens.
This process was repeated twice, after which the cytosol was treated as free of endogenous arginine.
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It is reasonable to expect overexpression of LdUba5C217S would have a negative dominant effect which will result in depletion of the free pool of endogenous Ufm1.
H&E-stained aortic sections were imaged using two nonlinear optical (NLO) microscopy techniques, two-photon excitation fluorescence (TPEF), and second harmonic generation (SHG) for label-free imaging of endogenous macromolecules.
Chain perdeuteration of CER shifts the methylene stretching frequencies to a spectral region free from interference of endogenous skin vibrations.
The typical chromatograms of blank rat plasma, the LLOQ sample and in vivo plasma sample after oral administration of YH-8 are presented in Fig. 2. Compared to spiked plasma samples, all blank plasma samples from six individual rats were found to be free from interference of endogenous substances at the retention time of YH-8 in the SRM scan mode.
Recombinant kinase activity of p38a from optimized constructs was determined as well, resulting in in vitro phosphorylated substrate ATF-2, but could not be separated free of doubt from endogenous kinase activity (data not shown).
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