Overall, the evaluated miniaturized spin column procedure enabled rapid loss-free extraction of unconjugated mycotoxins from conventional grain matrix and a significantly reduced matrix load in the extracts.
Solid phase microextraction (SPME) is a widely accepted solvent-free extraction technique that usually uses a polymer sorbent as the extraction phase.
The mechanical reliability of the bioactive glass films applied to real Ti dental implant fixtures has been evaluated by a procedure comprised of "cold" implantation in pig mandibular bone from a dead animal, followed by immediate tension-free extraction tests.
Recent evaluations of the MagNA Pure LC (MP; Roche Applied Science, Indianapolis, IN) suggest that it is suitable for automated, contamination-free extraction and purification of viral nucleic acids from large-volume (1.0 mL) serum or plasma specimens.
Samples were washed in 1 ml of O2-free extraction buffer then transferred to a 15-ml conical tube.
Media was removed; cells were frozen at −80°C for 15 min; transferred to vacuum and incubated 1 h, 25°C with 1 ml of O2-free extraction buffer (50 mM NaHPO4, pH 7.0, 1 mM EDTA, 10 mM NEM [N-ethyl maleimide], 10 mM IAA [iodoacetic acid], 1% Triton X-100, 5 mM NaF, 50 µg/ml leupeptin and 50 µg/ml aprotinin).
First, the quartz filters were transferred into pyrogen-free extraction tubes with 4 mL pyrogen-free water.
Although a handful of tools have recently been developed to meet this need, none of them provide error-free extraction of highly detailed information.
In this work, we have demonstrated the impact of membrane electrolysis, a chemical-free extraction technology, on the fermentation of an under-utilized, real biorefinery stream to generate reduced VFA.
Differences between SDS-free and SDS-containing sample extraction affected the relative content of membrane proteins identified, which was higher in the latter, whereas the native (SDS-free) extraction resulted in a higher number of proteins identified in total.
Total human DNA was isolated from placentas of healthy donors using a phenol-free extraction method and fragmented down to the average size of 0.3 6 kb using a sonifier set at 22 kHz.
