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But this narrative foreplay isn't just play, because it is forced and not free, conditioned by Iranian political reality.
Owing to the technical and practical limitations of cell therapy, cell free conditioned media may represent a potent alternative for therapeutic angiogenesis in ischemic cardiovascular diseases.
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Initially serum-free conditioned media from type I astrocytes (astrocyte conditioned medium) (Noble and Murray, 1984) was found to be mitogenic for neonatal rat OECs but this proliferative capacity is short-lived (Alexander et al., 2002).
Serum-free conditioned media.
For purification, the periplasmic fraction from transformed E. coli BL21 DE3) cells and the serum-free conditioned media from stably transfected HEK-293 cells were independently collected.
Serum-free conditioned media (SFCM) were collected from confluent cultures at 24 h, clarified by centrifugation and concentrated by 10-fold using centrifugal filter devices (Amicon, MWCO of 10 kDa).
Zymography assay was done on serum-free conditioned media derived from control cells or cells stably transfected with α1-PDX [25], [32].
Serum-free conditioned media were analyzed on 10% SDS-PAGE gels containing either 1 mg/mL gelatin for gelatinase activity or 2 mg/mL casein (Sigma) and 10 µg/mL plasminogen (Calbiochem) for uPA activity.
To determine if diffusible factors secreted by muscle cells induce cell cycle arrest and/or cell death in B16 cells, we collected serum-free conditioned media from C2C12 muscle cultures (CMC2C12) and from 10T1/2 fibroblast cultures (CM10T1/2).
Serum-free conditioned media was collected after 48 h and passed through a 0.45 μm filter.
Cell-free conditioned media was collected and stored at −80°C until required.
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