For HPLC analysis, at least 50 μg of protein was dried by centrifugation under vacuum, reconstituted with metal-free concentrated HCl (SeaStar Chemicals), and incubated overnight at 95 °C for protein hydrolysis.
For example, of four lots of a brand of lactose-free concentrated liquid formula from one company, one contained 2.04 ng g−1 Cd, while the other three contained 0.07 0.15 ng g−1.
Bacteria-free concentrated supernatants from M. haemolytica culture was subjected to LC MS/MS.
For analysis of proMMP-2 activation, conditioned medium was collected after 24 hours in serum-free medium, concentrated, and analyzed for gelatinase activity by zymography.
Two separate phases were obtained in the extract, demonstrating that CC-SFF of aqueous apple aromas is highly capable of producing a water-free super-concentrated product.
Its tunes arise from pools of crazy energy, and its concentrated free patches feel directed by strategy and language, some kinetic, some quiet and spacious.
Supernatants were tested at three concentrations; concentrated 5-fold (Vivaspin columns, Sartorius Stedim UK Ltd ,Surrey, UK), neat and diluted 3-fold in serum-free medium.
CM were dialyzed against serum-free medium and concentrated 10-fold by lyophilisation.
The serious unresolved paradox that the torsional angle of a clamped-free FG nanotube with a concentrated torque at free end, when being modeled using the nonlocal differential model, is found to be identical with the classical result can now be well resolved based on the developed nonlocal integral model.
Absolute progenitor (CFU-C) numbers at the end of transduction were higher in serum-free + concentrated virus transductions, as opposed to transductions in standard vector supernatants containing fetal calf serum.
Along with other interventions, the team provided free chlorine as a concentrated liquid through prominently displayed dispensers at local water sources.
